The viable separated circulating tumor cells can be possibly used for downstream molecular characterization. MetaCell® provides the possibility of single cell gene expression analysis or subsequent in vitro testing of chemoresistance.
Generally, the CTCs exhibit blobbed surface both in the „human“ CTCs-cultures and experimental animal CTCs in vitro cultures . The prominent vesicles could serve as communicators between the different cell groups. The communication through vesicles (most probably kind of exosomes) has been proven by confocal microscopy and could be a part of CTCs- heterogeneity evolution.
MetaCell® technology tends to develop means to determine the molecular profile of cancer cells in circulation. The molecular profile will reflect the sensitivity of the particular clones in the presence of various drugs, and could guide treatment decisions.